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知無(wú)不“研“ | ChIP實(shí)驗(yàn)基礎(chǔ)攻略
*流感數(shù)據(jù)共享計(jì)劃或?qū)椭钟祟惛腥拘约膊〉谋l(fā)
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VGF神經(jīng)生長(zhǎng)因子誘導(dǎo)蛋白(VGF)重組蛋白 VGF是神經(jīng)元合成和分泌的肽,在PC12細(xì)胞系中被NGF上調(diào)。VGF在PNS和CNS中廣泛表達(dá),但在成人下丘腦中尤其豐富。VGF在大腦調(diào)節(jié)能量消耗和體重方面起著至關(guān)重要的作用。損傷、生物鐘和神經(jīng)元活動(dòng)會(huì)迅速誘導(dǎo)其表達(dá)
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品牌 | 信裕生物 | CAS | 詳見說明書 |
---|---|---|---|
分子式 | 詳見說明書 | 純度 | > 97% |
分子量 | 43.3kDa | 貨號(hào) | XY95320RPHu01 |
規(guī)格 | 50µg/100µg | 供貨周期 | 一周 |
主要用途 | SDS-PAGE; WB | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
Recombinant VGF Nerve Growth Factor Inducible (VGF)
VGF神經(jīng)生長(zhǎng)因子誘導(dǎo)蛋白(VGF)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Asp330~Pro449
Tags: N-terminal His and GST Tag
Subcellular Location: Secreted
Purity: > 97%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% SKL, 5% Trehalose and Proclin300.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 4.8
Predicted Molecular Mass: 43.3kDa
Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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