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知無(wú)不“研“ | ChIP實(shí)驗(yàn)基礎(chǔ)攻略
*流感數(shù)據(jù)共享計(jì)劃或?qū)椭钟祟惛腥拘约膊〉谋l(fā)
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NCALd抗原;神經(jīng)鈣蛋白δ(NCALd)重組蛋白多種屬 該基因編碼神經(jīng)元鈣傳感器(NCS)鈣結(jié)合蛋白家族的成員。該蛋白含有一個(gè)N-末端肉豆蔻?;盘?hào)和四個(gè)EF手鈣結(jié)合環(huán)。該蛋白質(zhì)在靜息鈣水平下是細(xì)胞質(zhì)蛋白;然而,升高的細(xì)胞內(nèi)鈣水平會(huì)引起構(gòu)象變化,暴露肉豆蔻?;?,導(dǎo)致蛋白質(zhì)與膜結(jié)合,并與核周跨高爾基網(wǎng)絡(luò)部分共定位。該蛋白質(zhì)被認(rèn)為是G蛋白偶聯(lián)受體信號(hào)轉(zhuǎn)導(dǎo)的調(diào)節(jié)因子。已經(jīng)確定了該基因的幾個(gè)選擇性剪接變
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品牌 | 信裕生物 | CAS | 詳見說明書 |
---|---|---|---|
分子式 | 詳見說明書 | 純度 | > 90% |
分子量 | 25.8kDa | 貨號(hào) | XY93459RPHu |
規(guī)格 | 50µg/100µg | 供貨周期 | 一周 |
主要用途 | SDS-PAGE; WB | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
Recombinant Neurocalcin Delta (NCALd)
神經(jīng)鈣蛋白δ(NCALd)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Gly2~Phe193
Tags: N-terminal His Tag
Subcellular Location: Cytoplasm
Purity: > 90%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.2
Predicted Molecular Mass: 25.8kDa
Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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