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HSP90aA1抗原;90kDa熱休克蛋白αA1(HSP90aA1)重組蛋白多種屬 (熱休克蛋白90)是一種分子伴侶,是無(wú)應(yīng)激細(xì)胞中含量的蛋白質(zhì)之一。它是一種普遍存在的分子伴侶,存在于真細(xì)菌和真核生物的所有分支中,但在古細(xì)菌中顯然不存在。盡管細(xì)胞質(zhì)Hsp90在真核生物的所有條件下都是生存所必需的,但細(xì)菌同源物HtpG在非熱應(yīng)激條件下是不必要的。
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品牌 | 信裕生物 | CAS | 詳見(jiàn)說(shuō)明書(shū) |
---|---|---|---|
分子式 | 詳見(jiàn)說(shuō)明書(shū) | 純度 | > 95% |
分子量 | 62.9kDa | 貨號(hào) | XY92349RPHu01 |
規(guī)格 | 50µg/100µg | 供貨周期 | 一周 |
主要用途 | SDS-PAGE; WB | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
Recombinant Heat Shock Protein 90kDa Alpha A1 (HSP90aA1)
90kDa熱休克蛋白αA1(HSP90aA1)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Met1~Gly496 linked with Ile698~Asp732
Tags: N-terminal His Tag
Subcellular Location: Cytoplasm
Purity: > 95%
Traits: Freeze-dried powder
Buffer formulation: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%Trehalose.
Original Concentration: 2000µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 4.8
Predicted Molecular Mass: 62.9kDa
Accurate Molecular Mass: 72kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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