氯化物細(xì)胞內(nèi)通道蛋白1(CLIC1)重組蛋白

氯化物細(xì)胞內(nèi)通道蛋白1(CLIC1)重組蛋白氯通道是一組不同的蛋白質(zhì)，調(diào)節(jié)基本的細(xì)胞過(guò)程，包括細(xì)胞膜電位的穩(wěn)定、跨上皮轉(zhuǎn)運(yùn)、細(xì)胞內(nèi)pH值的維持和細(xì)胞體積的調(diào)節(jié)。氯化物細(xì)胞內(nèi)通道1是p64家族的成員；該蛋白主要定位于細(xì)胞核，并表現(xiàn)出核膜和質(zhì)膜氯離子通道活性?！居蒖efSeq提供，2008年7月】

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詳細(xì)介紹

品牌	信裕生物	CAS	詳見(jiàn)說(shuō)明書
分子式	詳見(jiàn)說(shuō)明書	純度	> 98%
分子量	21.9kDa	貨號(hào)	XY90975RPHu01
規(guī)格	50µg/100µg	供貨周期	一周
主要用途	SDS-PAGE; WB; ELISA; IP	應(yīng)用領(lǐng)域	生物產(chǎn)業(yè)

Recombinant Chloride Intracellular Channel Protein 1 (CLIC1)

氯化物細(xì)胞內(nèi)通道蛋白1(CLIC1)重組蛋白

[ PROPERTIES ]

Source:Prokaryotic expression

Host: E.coli

Residues:Lys79~Lys241

Tags:N-terminal His Tag

Tissue Specificity:Heart, Liver, Kidney, Pancreas.

Subcellular Location: Nucleus. Nucleus membrane; Single-pass membraneprotein Probable. Cytoplasm. Cell membrane.

Purity: > 98%

Traits:Freeze-dried powder

Buffer formulation: 100mM NaHCO₃, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration:200µg/mL

Applications:SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:5.8

Predicted Molecular Mass:21.9kDa

Accurate Molecular Mass:26kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO₃, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

氯化物細(xì)胞內(nèi)通道蛋白1(CLIC1)重組蛋白